Regulatory

Part:BBa_K4628019:Design

Designed by: Linghao Xia   Group: iGEM23_ZJU-China   (2023-10-11)


subPromoter MP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because part of the movement protein coding sequence is included in the TMV RNA-dependent RNA polymerase coding sequence, it means that the retention of intact replicases and subgenomic promoters may result in meaningless partial movement protein peptide chains being transcribed and translated, affecting the correct folding and functional expression of the target protein. However, our results successfully prove that it is feasible to express GFP with this promoter without affecting its normal luminescence


Source

Tobacco mosaic virus genome (NCBI Reference Sequence: NC_001367.1)

References